| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | The beads were eluted with an elution buffer containing 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.03% DDM, 0.003% CHS, and 250 mM imidazole, and further incubated for 2 h with anti-DYKDDDDK G1 Affinity Resin (GenScript) to improve protein purity. The protein sample was then eluted by DYKDDDDK peptide (GenScript) and purified by size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 GL column (GE Healthcare), equilibrated with a SEC buffer containing 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.03% DDM, and 0.003% CHS. | Get A Quote |
Lactate metabolism and signaling intricately intertwine in the context of cancer and immunity. Basigin, working alongside monocarboxylate transporters MCT1 and MCT4, orchestrates the movement of lactate across cell membranes. Despite their potential in treating formidable tumors, the mechanisms by which basigin antibodies affect basigin and MCTs remain unclear. Our research demonstrated that basigin positively modulates MCT activity. We subsequently developed a basigin antibody that converts basigin into a negative modulator, thereby suppressing lactate transport and enhancing anti-tumor immunity. Additionally, the antibody alters metabolic profiles in NSCLC-PDOs and T cells. Cryo-EM structural analysis and mol... More
