Whole tumor cell vaccines have shown much promise， but demonstrated poor efficiency in phase III trials. In this study， we modified MDA-MB‑231 tumor cells (MDA-MB‑231Gal+) to express α-1， 3-galactosyltransferase (α-1， 3-GT) protein， to potentially enhance antitumor effect of whole tumor cell vaccines. MDA-MB‑231 tumor cell vaccines were transfected with a reconstructed lentiviral containing α-1， 3-GT genes. Tumor growth， tumorigenesis and survival of Hu-NOD-SCID mice were observed when tumor-bearing mice were injected with tumor cell vaccines. Proliferation and apoptosis in MDA-MB‑231 tumor xenografts were observed by immunohistochemistry. The levels of cytokine secretion in the serum ... More
Whole tumor cell vaccines have shown much promise， but demonstrated poor efficiency in phase III trials. In this study， we modified MDA-MB‑231 tumor cells (MDA-MB‑231Gal+) to express α-1， 3-galactosyltransferase (α-1， 3-GT) protein， to potentially enhance antitumor effect of whole tumor cell vaccines. MDA-MB‑231 tumor cell vaccines were transfected with a reconstructed lentiviral containing α-1， 3-GT genes. Tumor growth， tumorigenesis and survival of Hu-NOD-SCID mice were observed when tumor-bearing mice were injected with tumor cell vaccines. Proliferation and apoptosis in MDA-MB‑231 tumor xenografts were observed by immunohistochemistry. The levels of cytokine secretion in the serum of mice were tested by ELISA. CD8+ T cells infiltrating tumors were assessed by flow cytometry. MDA-MB‑231Gal+ cells expressed active α-1， 3-GT and produced α-Gal in vitro. MDA-MB‑231Gal+ cell vaccines suppressed tumor growth and tumorigenesis in immunized Hu-NOD-SCID mice. Additionally， decrease of TGF-β， IL-10 and increase of INF-γ， IL-12 were observed in tumor cell vaccinated mice. Furthermore， the cell vaccines enhanced infiltration of cytotoxic CD8+ T cells in the tumor microenvironment of immunized mice. The MDA-MB‑231Gal+ cell vaccines modified α-1， 3-GT genes improved the antitumor effect.
