| ￥3000 | |
| Z05791-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 45.8 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind RNF43 hFc Chimera, Human, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay (Gator® Prime). »
Immobilized Human R-Spondin 3, His Tag at 2 μg /ml(100 μl/well) on the plate. Dose response curve for RNF43 hFc Chimera, Human, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. »
Immobilized Human R spondin 1 at 5 μg /ml(100 μl/well) on the plate. Dose response curve for RNF43 hFc Chimera, Human, hFc Tag with the EC50 of 0.4 μg /ml determined by ELISA. »
The purity of RNF43 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
RNF43 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation. |
| Synonyms | E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
