| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 68.29 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized G-CSF R/CD114, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. »
The purity of G-CSF R/CD114, His, Human is greater than 95% as determined by SEC-HPLC. »
G-CSF R/CD114, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | The Granulocyte Colony-Stimulating Factor (G-CSF) receptor, a member of the hematopoietin cytokine receptor superfamily, functions as a homodimer and requires the recruitment of cytosolic protein tyrosine kinases (PTKs) to transduce its signal. |
| Synonyms | G-CSF-R; G-CSF R; CD114; G CSF R; CSF3R; Csfgr; GCSFR |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.