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DKK1[Biotin], His & Avi, Human

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
￥5800
Z04440-100

产品简介
Species Human
Protein Construction
DKK1 (Thr32-His266)
Accession # O94907		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 28.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
DKK1[Biotin], His & Avi, Human

Immobilized DKK1[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human LRP-5, hFc Tag with the EC50 of 50.4 ng/ml determined by ELISA. »

DKK1[Biotin], His & Avi, Human

Immobilized DKK1[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.34 μg/ml determined by ELISA. »

DKK1[Biotin], His & Avi, Human

Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for DKK1[Biotin], His & Avi, Human, His Tag with the EC50 of 3.1 ng/ml determined by ELISA. »

DKK1[Biotin], His & Avi, Human

The purity of DKK1[Biotin], His & Avi, Human is greater than 90% as determined by SEC-HPLC. »

DKK1[Biotin], His & Avi, Human

DKK1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Synonyms Dickkopf-1; Dkk1; SK
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.